Detection of Tobacco streak virus infecting sunflower by ELISA and RT-PCR method
نویسندگان
چکیده
منابع مشابه
Reproducibility in the quantification of mRNA levels by RT-PCR-ELISA and RT competitive-PCR-ELISA.
The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...
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A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies tha...
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Avian leukosis viruses (ALVS) cause different types of tumours in poultry and can affect the health and egg production of the birds. To investigate the presence of the virus in chicken layer flocks in Shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...
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Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
متن کاملThe use RT - PCR for detection of viruses infecting cucumber
Cucumber plants exhibiting virus-like disease symptoms were collected in various gardens and greenhouses in Lithuania. Viral diseases were expressed by symptoms of mosaicmottling, yellow ringspots and vein necrosis of leaves. Growth and fruiting of plants were reduced. Results of the identification of viruses detected in cucumbers by the RT-PCR technique are presented here. Identification of ca...
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ژورنال
عنوان ژورنال: INTERNATIONAL JOURNAL OF PLANT PROTECTION
سال: 2015
ISSN: 0974-2670,0976-6855
DOI: 10.15740/has/ijpp/8.1/99-103